Background and Objectives: Metabolic perturbations frequently occur during the acute phase of critical illness and may disrupt lipid homeostasis. Acute respiratory distress syndrome (ARDS) is a severe condition and is associated with high mortality in critically ill patients with sepsis. This study aimed to evaluate the association between conventional plasma lipid parameters and clinical outcomes in critically ill sepsis patients. Materials and Methods: We conducted a prospective observational...

To provide evidence for HIV-1 DNA genotypic resistance testing (GRT) strategies in low-level viremia (LLV) populations by comparing the amplification efficiency and resistance mutation profiles between HIV-1 DNA GRT and RNA GRT. (2) Methods: Convenience sampling was used for participant enrolment. Plasma HIV-1 RNA and peripheral blood monocyte (PBMC) DNA were extracted separately. The protease-reverse transcriptase (PR-RT) region was amplified via an in-house protocol, followed by Sanger and...

Sepsis‑associated acute kidney injury (S‑AKI) is a major cause of morbidity and mortality in critically ill patients, yet effective targeted therapies remain lacking due to an incomplete understanding of its pathogenesis. Ferroptosis, an iron‑dependent form of regulated cell death driven by lipid peroxidation, has recently been implicated in AKI, but the upstream regulators of this process in sepsis remain undefined. Here, we identify SIRT5, a mitochondrial NAD ⁺ ‑dependent desuccinylase, as a...

CONCLUSIONS: First, the sequential transformation from early pyroptosis and NETosis (driving hyperinflammation) to mid-stage necroptosis (amplifying tissue damage) to late lymphocyte apoptosis (mediating immunosuppression), with distinct cellular responses in immune cells versus parenchymal tissues. Second, we have distilled complex pathway interactions into three converging decision nodes, RIPK1 ubiquitination status, caspase-8 activity, and membrane pore executors (GSDMD/MLKL/NINJ1), whose...