The pathogenicity of Pseudomonas aeruginosa in bloodstream infection is shaped by biofilm formation and the type III secretion system (T3SS). We characterised the biofilm-associated and T3SS genotypes of 100 non-duplicate bloodstream isolates and tested whether either trait co-segregates with antimicrobial resistance. Biofilm was assessed by Congo red agar (CRA) and the quantitative microtiter plate (MTP) assay; biofilm-associated (pslA, pelA, ppyR) and T3SS effector (exoS, exoY, exoT, exoU)...

CONCLUSIONS: AMR within this surveillance network did not follow a universal upward curve; it evolved through pathogen-specific architectures of burden, acceleration, redistribution, and therapeutic-space contraction. Integrating these dimensions distinguished entrenched high-volume reservoirs from rapidly deteriorating invasive or lower-frequency threats that cumulative antibiograms may overlook. This network-based framework offers a potential early-warning approach for identifying...

(1) Background: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli are important causes of bacteremia, but integrated genotype-virulence-outcome data from low- and middle-income settings are limited; (2) Methods: Observational study of ESBL-producing E. coli (ESBL Ec) bacteremia at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia (2022-2024). ESBL production was confirmed phenotypically and ESBL genes by PCR; eight ExPEC-associated virulence genes (fimH, hlyA, iutA,...

Getah virus (GETV) is a mosquito-borne Old World alphavirus that has recently increased its geographic range, particularly in China. GETV causes rheumatic disease in horses, but in recent years its primary impact has been lethal infections in newborn piglets. Herein, we characterize a lethal Ifnar1-/- mouse model of GETV which recapitulates many of the features seen in piglets. These include lethality, high viremia, diarrhoea, and splenomegaly, with histopathological findings aligning with those...